Reporter
mRFP1

Part:BBa_K2835001:Design

Designed by: Hanna Norbäck, Emma Larsson   Group: iGEM18_Stockholm   (2018-08-21)


Constitutive reporter: strong promoter + RBS + mutant of mRFP1 (RFC25 compatible)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Assembly: Strong constitutive promoter + strong RBS + mutant of mRFP1 (AgeI sites removed)

Source

The RFP gene is originally derived from Discosoma striata (coral). The mRFP1 gene is originally derived from a monomeric derived version of RFP, GenBank: AF506027.1

References